Koizumi I, Suzuki Y, Shimura S. Deodorant effects of champignon extract and repressive effects on production of indole and tryptamine in vivo. Nihon Koshu Eisei Zasshi. 1997; 44(1):5-11.

Topic: Does Champex reduce body odor in pets?

Background: Offensive oral and fecal odors are often generated when meat, fish, and shellfish become putrid. These foods are decomposed by fermentation in the bowel producing indole, ammonia, and skatol, which are all compounds associated with foul odor. This study investigates the ability of Champex, a Champignon mushroom extract, to reduce odor using in vitro and in vivo methodologies.

Study Design: In vitro methodology: 1g of Chicken liver homogenate (CLH) mixed with 9mL distilled water served as control samples. Experimental samples consisted of 1g CLH mixed with 0.5mL, 1.0mL, 2.0mL, and 4.0mL Champexas well as an addition of distilled water to achieve final sample volume of 9mL. Samples were incubated at 37°C. At time points 6 hours, 12 hours, 24 hours, 48 hours, and 72 hours, the samples were assayed for ammoniacal nitrogen to determine the repressive effect of Champex on production of ammonia during decomposition. All experiments were performed in triplicate.

In vivo methodology: 25 male Japanese white native rabbits with weights ranging from 2.0-2.5kg were utilized as model organisms. Food and water were provided ad libitum for 15 days. 21 of these individuals were divided into 3 experimental groups. Group 1: Control group (n=5). Group 2: Administered 1.0g/ kg BW tryptophan and 10mL water (n=5). Group 3: Administered tryptophan 1.0g/kg BW and Champex 5mL/kg BW. In addition to these treatments food and water were provided ad libitum. 1mL blood samples were taken from each rabbit at time points of 1 hour, 3 hours, 6 hours, 9 hours, and 12 hours following administration of experimental treatment. Subsequent extraction and HPLC analysis was performed on samples to ascertain concentrations of tryptophan, tryptamine, and indoacetic acid.

Results: In vitro experiment: At time points ≥24 hours, Champex showed a dose dependent reduction in ammoniacal nitrogen generated from the putrefaction of chicken liver homogenate.

In vivo experiment: At time points t=6 hours, serum from individuals in group 3 showed significantly lower concentrations of tryptophan. At time points t≥3 hours, serum from individuals in group 3 showed significantly lower concentrations of indoleacetic acid. At each time point, serum from individuals in group 3 showed no tryptamine. Therefore, group 3 showed significantly lower concentrations of tryptamine at time points t=1 hour, 3 hours, 6 hours compared to control.